Phospho-GSK3A/GSK3B (Y216 + Y279) Antibody

IHC image of CSB-RA009962A216phHU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4?C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.