Acetyl-HIST1H1C (K74) Antibody

Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with CSB-PA010378OA74acHU at 1:7.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4?C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).